Composite

Part:BBa_K1453405:Design

Designed by: Yangyang Li, Binhan Hao, Renhe Luo   Group: iGEM14_SJTU-BioX-Shanghai   (2014-10-17)


ssDsbA-Lgt-poxB-TAL1-His Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 862
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 483
    Illegal BsaI.rc site found at 3274
    Illegal BsaI.rc site found at 3886


Design Notes

This part can recognize a special sequence, T-TCGATATCAAGC-T. This part contains the pyruvate dehydrogenase (quinone) [EC:1.2.5.1]


Source

E.coli, BBa_K1453403, BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089.

References